Oscar Polk
Gabriel
Eddie 'Rochester' Anderson
Noah
Rex Ingram
De Lawd / Adam / Hezdrel
Frank H. Wilson
Moses
George H. Reed
Mr. Deshee / Aaron
Abraham Gleaves
Archangel
Myrtle Anderson
Eve
Al Stokes
Cain
Edna Mae Harris
Zeba
James Fuller
Cain the Sixth
George Randol
High Priest
Ida Forsyne
Noah's Wife
Anna Mae Fritz
Stout Angel / Young Gambler / Second Cleaner (uncredited)
Etta Moten
Angel (uncredited)
Bill Cumby
Abraham / Head Magician / King of Babylon
Clarence Muse
Angel (uncredited)
Fred 'Snowflake' Toones
Zubo (uncredited)
Ernest Whitman
Pharaoh
Willie Best
Henry - the Angel (uncredited)
Dudley Dickerson
Ham
Etta McDaniel
Heckler at the Ark (uncredited)
Mantan Moreland
Angel Removing Hat (uncredited)
Jester Hairston
Member of Hall Johnson Choir (uncredited)
Theresa Harris
Angel (uncredited)
Jesse Graves
General (uncredited)