James Stewart
Paul "Pauly" Biegler
Lee Remick
Laura Manion
Ben Gazzara
Lieutenant Frederick "Manny" Manion
Arthur O'Connell
Parnell Emmett "Parn" McCarthy
Eve Arden
Maida Rutledge
Kathryn Grant
Mary Pilant
George C. Scott
AAG Claude Dancer
Orson Bean
Dr. Matthew Smith
Russ Brown
Deputy Sheriff George Lemon
Murray Hamilton
Alphonse "Al" Paquette
Brooks West
DA Mitchell "Mitch" Lodwick
Ken Lynch
Det. Sgt. James "Jim" Durgo
John Qualen
Deputy Sheriff Sulo Kangas
Howard McNear
Dr. Adelord Dompierre
Alexander Campbell
Dr. W. Gregory Harcourt
Ned Wever
Dr. Homer Raschid
Jimmy Conlin
Clarence "One-Shot" "Smoky" Madigan
Joseph N. Welch
Judge Harlan Weaver
Royal Beal
Sheriff Max Battisfore
Joseph Kearns
Lloyd Burke
Don Ross
Duane "Duke" Miller
Lloyd Le Vasseur
Court clerk
James Waters
Army Sergeant 1st Class
Duke Ellington
Pie Eye (uncredited)
Irv Kupcinet
Distinguished Gentleman (uncredited)
Chuck Ramsay
Orderly (uncredited)
Agnes Rodgers Brown
Juror (uncredited)