Milla Jovovich
Alice
Oded Fehr
Carlos Olivera
Ali Larter
Claire
Iain Glen
Dr. Isaacs
Ashanti
Betty
Christopher Egan
Mikey
Spencer Locke
K-Mart
Matthew Marsden
Slater
Linden Ashby
Chase
Jason O'Mara
Albert Wesker
Mike Epps
L.J.
Joe Hursley
Otto
John Eric Bentley
Umbrella Tech
James Tumminia
Lab Tech
Kirk B.R. Woller
Scientist
Rick Cramer
Ice Hockey / Corridor Guard
Madeline Carroll
White Queen
Peter O'Meara
British Envoy
William Abadie
French Envoy
Ramón Franco
Runty
Shane Woodson
Piggy
Valorie Hubbard
Ma
Geoff Meed
Pock Mark
Rusty Joiner
Eddie
Brian Steele
Rancid / Tyrant
Connor McCoy
Small Boy
Gary A. Hecker
Tyrant Vocal (voice)
Richard Epcar
Additional Voices (voice) (uncredited)
Gary Hudson
Umbrella Captain (uncredited)
Carsten Nørgaard
Zombie Steve (uncredited)
César Ámigo Aguilar
Foreign Envoy (uncredited)